Research and development specialist

Micro Crispr Pvt. Ltd. · Vapi, India

Company Profile

Micro CRISPR is a pioneering biotechnology company based in Vapi, Gujarat, India, focused on advancing the field of synthetic biology and precision genome engineering. Founded in 2021, the company leverages state-of-the-art CRISPR gene-editing technologies to develop novel “living therapeutics” aimed at addressing major human health challenges, including cancer, metabolic and genetic disorders, neurodegeneration, and infectious diseases. Its research initiatives span advanced cell and gene therapies such as autologous and allogenic CAR-T platforms, customised m RNA vaccines, and sustainable biotechnology solutions for agriculture and other sectors. With a strong R&D team and mission to improve millions of lives through bold innovation and responsible scientific development, Micro CRISPR is committed to shaping the future of medicine and biotechnology.

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Job Summary

We are seeking a highly motivated Research & Development Scientist to support the research and development of CAR-T Cell therapies. The ideal candidate should have hands-on experience with primary immune cell culture, CAR-T cell generation, molecular biology techniques, functional assays, and analytical method development. The candidate will contribute to developing novel CAR-T products from early discovery through preclinical development.

Key Responsibilities Design and execute experiments related to CAR-T cell development. Isolate, activate, culture, expand, and characterize primary human T cells/PBMCs. Perform mammalian and primary cell culture under sterile conditions. Conduct viral transduction (Lentivirus/Retrovirus) for CAR expression. Perform analytical method development and qualification for CAR-T characterization. Develop and execute cytotoxicity/potency assays. Perform molecular biology techniques including: PCR, q PCR / RT-q PCR / dd PCR (preferred) Perform flow cytometry-based immunophenotyping and CAR expression analysis. Execute cell viability, proliferation, apoptosis and cytokine assays. Analyze experimental data and prepare technical reports. Prepare SOPs, study protocols and scientific documentation. Collaborate with cross-functional teams including Process Development, Analytical Development and Manufacturing.

Required Technical Skills Strong experience with Primary Human Cell Culture PBMC isolation and T-cell activation/expansion CAR-T Cell culture and characterization Mammalian Cell Culture Cell-based Assays Cytotoxicity Assays PCR / q PCR / RT-PCR Flow Cytometry ELISA Cell Viability Assays Molecular Biology Techniques Analytical Method Development Data Analysis GLP/GMP documentation (preferred)

Preferred Experience CAR-T Cell Therapy Cell & Gene Therapy Immunology Oncology Research Viral Vector (Lentivirus/AAV) Gene Editing (CRISPR) – desirable Potency assay development Technology transfer experience (preferred)

Education MSc / MTech / Ph D in Biotechnology, Immunology, Cell Biology, Molecular Biology, Life Sciences or related field.

Experience Scientist: 3–6 years Experience in CAR-T, Cell Therapy or Immunology R& D preferred.